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Instructor-led course

Provided by: Bioinformatics


This course has 1 scheduled run. To book a place, please choose your preferred date:


Wed 15 Nov 2017


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Analysis of DNA Methylation using Sequencing
Beginners


Description

This course will cover all aspects of the analysis of DNA methylation using sequencing, including primary analysis, mapping and quality control of BS-Seq data, common pitfalls and complications.

It will also include exploratory analysis of methylation, looking at different methods of quantitation, and a variety of ways of looking more widely at the distribution of methylation over the genome. Finally the course will look at statistical methods to predict differential methylation.

The course will be comprised of a mixture of theoretical lectures and practicals covering a range of different software packages.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book by linking here.

Target audience
  • Graduate students, Postdocs and Staff members from the University of Cambridge, Affiliated Institutions and other external Institutions or individuals
  • Please be aware that these courses are only free for University of Cambridge students. All other participants will be charged a registration fee in some form. Registration fees and further details regarding the charging policy are available here.
  • Further details regarding eligibility criteria are available here
Topics covered

Bioinformatics, Data visualisation, Data handing, Epigenetics

Objectives

After this course you should be able to:

  • Perform an analysis of methylation data all the way from raw sequencing to the selection of interesting targets
  • Understand BS-Seq theory and quality control
  • Use SeqMonk to visualise and analyse methylation data
Aims

During this course you will learn about:

  • The theoretical basis for BS-Seq
  • Processing raw sequencing data with bismark
  • Visualisation and exploration of methylation calls with SeqMonk
  • The theory of differential methylation calling
  • Differential methylation analysis with SeqMonk
Format

Presentations, demonstrations and practicals

Timetable

Time Topics Speaker(s)
9:30 - 10:30 Bisulfite-Seq theory and QC Simon Andrews, Felix Krueger
10:30 - 11:15 Mapping and QC practical
11:15 - 11:30 Tea/Coffee Break
11:30 - 12:30 Visualising and Exploring talk
12:30 - 13:30 Lunch
13:30 - 14:00 Methylation tools in SeqMonk Simon Andrews, Felix Krueger
14:00 - 15:30 Visualising and Exploring practical
15:30 - 15:45 Tea/Coffee Break
15:45 - 16:35 Differential methylation talk & practical
16:35 - 17:00 Summary and Wrap-Up
Registration Fees
  • Free for University of Cambridge students
  • £ 50/day for all University of Cambridge staff, including postdocs, and participants from Affiliated Institutions. Please note that these charges are recovered by us at the Institutional level
  • It remains the participant's responsibility to acquire prior approval from the relevant group leader, line manager or budget holder to attend the course. It is requested that people booking only do so with the agreement of the relevant party as costs will be charged back to your Lab Head or Group Supervisor.
  • £ 50/day for all other academic participants from external Institutions and charitable organizations. These charges must be paid at registration
  • £ 100/day for all Industry participants. These charges must be paid at registration
  • Further details regarding the charging policy are available here
Duration

1 day

Frequency

A number of times per year

Related courses
Theme
Specialized Training

Events available