Analysis of DNA Methylation using Sequencing (IN-PERSON)

Beginners

This course will cover all aspects of the analysis of DNA methylation using sequencing, including primary analysis, mapping and quality control of BS-Seq data, common pitfalls and complications.

It will also include exploratory analysis of methylation, looking at different methods of quantitation, and a variety of ways of looking more widely at the distribution of methylation over the genome. Finally the course will look at statistical methods to predict differential methylation.

The course comprises of a mixture of theoretical lectures and practicals covering a range of different software packages.

If you do not have a University of Cambridge Raven account please book or register your interest here.

• ♿ The training room is located on the first floor and there is currently no wheelchair or level access.
• Our courses are only free for registered University of Cambridge students. All other participants will be charged according to our charging policy.
• Attendance will be taken on all courses and a charge is applied for non-attendance, including for University of Cambridge students. After you have booked a place, if you are unable to attend any of the live sessions, please email the Bioinfo Team.
• Further details regarding eligibility criteria are available here.
• Guidance on visiting Cambridge and finding accommodation is available here.

• Graduate students, Postdocs and Staff members from the University of Cambridge, Affiliated Institutions and other external Institutions or individuals

No previous bioinformatics experience is required, but an undergraduate level understanding of biology would be an advantage.

Bioinformatics, Data visualisation, Data handing, Epigenetics

After this course you should be able to:

• Perform an analysis of methylation data all the way from raw sequencing to the selection of interesting targets
• Understand BS-Seq theory and quality control
• Use SeqMonk to visualise and analyse methylation data

During this course you will learn about:

• The theoretical basis for BS-Seq
• Processing raw sequencing data with Bismark
• Visualisation and exploration of methylation calls with SeqMonk
• The theory of differential methylation calling
• Differential methylation analysis with SeqMonk

Presentations, demonstrations and practicals

• Free for registered University of Cambridge students
• £ 60/day for all University of Cambridge staff, including postdocs, temporary visitors (students and researchers) and participants from Affiliated Institutions. Please note that these charges are recovered by us at the Institutional level
• It remains the participant's responsibility to acquire prior approval from the relevant group leader, line manager or budget holder to attend the course. It is requested that people booking only do so with the agreement of the relevant party as costs will be charged back to your Lab Head or Group Supervisor.
• £ 60/day for all other academic participants from external Institutions and charitable organizations. These charges must be paid at registration
• £ 120/day for all Industry participants. These charges must be paid at registration
• Further details regarding the charging policy are available here

1

A number of times per year

• Introduction to R for Biologists (IN-PERSON)
• Analysis of bulk RNA-seq data (IN-PERSON)
• Analysis of single cell RNA-seq data (IN-PERSON)